Universität Bonn

Pharma-Zentrum Bonn

de
Dagmar Wachten
© B. Frommann, Uni Bonn
Prof. Dr. rer. nat. Dagmar Wachten
Faculty of Medicine

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Zugehörigkeiten
  • Institute for Innate Immunity
Forschungsschwerpunkte
  • cell-cell communication
  • primary cilia
  • cyclic nucleotides
  • optogenetics
  • tissue homeostasis
Primary cilia are considered as sensory organelles that receive extracellular signals from the environment and transduce this information into a cellular response. Whereas in motile cilia or flagella, e.g. of Paramecium, Chlamydomonas, airway epithelial cells, or mammalian sperm, cAMP regulates ciliary beating, the role of cAMP signaling in non-motile primary cilia is much less understood. Ciliary G-protein coupled receptors (GPCRs) are believed to act as extrasynaptic signaling devices, which sense the local environment. Upon stimulation, ciliary GPCRs have been proposed to locally change the cAMP concentration in the primary cilium, thereby, controlling cellular functions like gene expression. However, the cellular responses downstream of ciliary cAMP signaling and the physiological functions are rather enigmatic. We apply an interdisciplinary approach combining optogenetics, genetically-encoded biosensors, high-resolution imaging, cell biology, systems biology, and biochemistry.
Ausgewählte Publikationen

1.     Khani, S.*, Topel, H.*, Josephrajan, A., Larsen, B.D.M., Albuquerque de Almeida Tavenez, A.R., Gaudry, M.J., Leyendecker, P., Stanic, N., Gaziano, I., Hansmeier, N.R., Schmidt, E., Klemm, P., Vagliano, L.M., Engelhard, C.A., Nielsen, S., Jespersen, N.Z., Rehimi, R., Gohlke, S., Frommolt, P., Gnad, T., Rada-Iglesias, A., Pradas-Juni, M., Schulz, T.J., Wunderlich, F.T., Pfeifer, A., Jastroch, M., Wachten, D.#, Kornfeld, J.W.# Cold-induced expression of a truncated adenylyl cyclase 3 acts as a rheostat to brown fat function. Nat. Metabolism (2024) 6(6):1053-1075. doi: 10.1038/s42255-024-01033-8.

2.     Gopalakrishnan, J., Feistel, K., Friedrich, B.M., Grapin-Botton, A., Jurisch-Yaksi, N., Mass, E., Mick, D.U., Müller, R.U., May-Simera, H., Schermer, B., Schmidts, M., Walentek, P., Wachten, D. Emerging principles of primary cilia dynamics in controlling tissue organization and function. EMBO J. (2023) 42(21):e113891.  doi: 10.15252/embj.2023113891.

3.     Hansen, J.N., Kaiser, F., Leyendecker, P., Stüven, B., Krause, J.H., Derakhshandeh G., S.F., Jaazba, I., Sroka, T.J., Preval, K.M., Desai, P.B., Kraut, M., Theis, H., Drews, A.D., De-Domenica, E., Händler, K., Pazour, G.J., Mick, D.U.,Wachten, D. A cAMP signalosome in primary cilia drives gene expression and kidney cyst formation. EMBO Rep.(2022), 43(5):366-378. doi: 10.1016/j.it.2022.03.001.

4.     Hansen, J.N., Raßmann, S., Stüven, B., Jurisch-Yaksi, N., Wachten, D. CiliaQ – a simple, open-source software for automated quantification of ciliary morphology and fluorescence in 2D, 3D, and 4D images. (2021) Eur. Phys. J. E. M., 44, 18

5.     Hansen, J.N., Kaiser, F., Klausen, C., Stüven, B., Chong, R., Bönigk, W., Möglich, A., Mick, D.U., Jurisch-Yaksi, N., Schmidt, F.I., Wachten, D. Nanobody-directed targeting of optogenetic tools reveals differential regulation of cilia length. (2020) eLife, 9:e57907, doi: 10.7554/eLife.57907.

Dagmar Wachten
© B. Frommann, Uni Bonn
Prof. Dr. rer. nat. Dagmar Wachten
Faculty of Medicine

Webseite

+49 228 287 51239